Table 4.
Plasma and serum oxidative stress and blood parameters (means ± s.d.) of lambs fed a high-concentrate diet with different antioxidants.
| Parameters | Treatment | Days | p-Value | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Control | FO | FO Plus SeE | SeE | 0 | 30 | 53 | Treatment | Time | Treatment * Time | |
| SOD | 0.261 ± 0.42 | 0.278 ± 0.34 | 0.375 ± 0.44 | 0.438 ± 0.47 | 0.410 ± 0.29 | 0.407 ± 0.37 | 0.197 ± 0.38 | 0.412 | 0.072 | 0.369 |
| GSH-Px | 373.12 ± 213 | 423.62 ± 235 | 356.57 ± 113 | 329.58 ± 150 | 375.56 ± 102 b | 488.14 ±149 a | 248.46 ± 132 c | 0.157 | <0.0001 | 0.006 |
| Catalase | 2.18 ± 2.26 | 2.34 ± 1.44 | 1.64 ± 2.79 | 2.46 ± 1.25 | 2.32 ±0.96 | 1.71 ±1.75 | 2.44 ± 1.49 | 0.531 | 0.353 | 0.852 |
| Peroxidases | 3.58 ± 1.37 b | 4.57 ± 1.27 ab | 8.11 ± 1.40 a | 2.65 ± 1.30 b | 5.56 ± 1.17 a | 2.23 ± 1.16 b | 6.40 ± 1.44 a | 0.035 | 0.031 | 0.539 |
| H2O2 | 932.56 ± 478 | 896.10± 181 | 891.83 ± 181 | 906.45 ± 247 | 988.38 ± 375 | 894.08 ± 261 | 837.74 ± 236 | 0.972 | 0.146 | 0.273 |
| ALT | 12.00 ± 4.81 a | 14.24 ± 6.54 a | 9.08 ± 5.74 b | 9.71 ± 3.93 b | 13.00 ± 4.93 | 10.46 ± 5.07 | 10.31 ± 5.16 | 0.006 | 0.095 | 0.935 |
| AST | 112.62 ± 32.54 a | 108.24 ± 35.83 a | 92.75 ± 14.58 b | 98.00 ± 25.78 b | 84.33 ± 10.34 c | 113.13 ± 20.14 ab | 111.25 ± 21.63 b | 0.033 | <0.0001 | 0.641 |
| ALP | 593.24 ± 208 | 608.79 ± 250 | 609.75 ± 165 | 600.29 ± 216 | 651.91 ± 187 | 605.49 ± 209 | 551.66 ± 209 | 0.990 | 0.193 | 1.000 |
| GGT | 87.03 ± 30.11 | 78.85 ± 30.29 | 75.54 ± 18.22 | 82.25 ± 35.39 | 59.79 ± 13.70 cc | 90.36 ± 19.96 ab | 92.59 ± 22.20 a | 0.447 | <0.0001 | 0.857 |
| Cholesterol | 62.75 ± 13.25 | 58.53 ± 15.05 | 53.90 ± 11.54 | 60.29 ± 14.47 | 49.87 ± 9.12 | 64.35 ± 10.19 ab | 62.38 ± 11.56 ab | 0.124 | <0.0001 | 0.114 |
SOD, superoxide dismutase; GSH-px, glutathione peroxidase; H2O2, hydrogen peroxide; ALT, alanine aminotransferase; AST, aspartate aminotransferase; ALP, alkaline phosphatase; GGT, gamma-glutamyl transferase. Control: without additives; FO: functional oils, 0.50 g/kg DM of castor and cashew nut shell oils; SeE: hydroxy-selenomethionine and vitamin E, 0.50 mg/kg of selenium and 100 IU/kg DM of vitamin E; FO plus SeE, at the same doses as the other groups. Means in the same row followed by different lowercase letters differ significantly at the 5% level.