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. 2020 Dec 18;21(24):9665. doi: 10.3390/ijms21249665

Figure 1.

Figure 1

SA-β-Gal activity and immunofluorescence microscopy of LAMP1 for pseudoxanthoma elasticum (PXE) fibroblasts and normal human dermal fibroblasts (NHDF). Fibroblasts were cultivated for 72 h in medium with 10% fetal calf serum (FCS) or 10% lipoprotein-deficient fetal calf serum (LPDS). (A) Qualitative senescence assay for PXE fibroblasts (n = 3) and NHDF (n = 3). Representative images are shown. Scale bar: 100 μm. (B) Quantitative senescence assay for PXE fibroblasts (gray, n = 3) and NHDF (white, n = 3). Data are shown as mean ± SEM. Control/ PXE: *** p ≤ 0.001. 10% FCS/10% LPDS: + p ≤ 0.05; ns p > 0.05. (C) Immunofluorescence microscopy of LAMP1 (green) for PXE fibroblasts (n = 3) and NHDF (n = 3). Cell nuclei were counterstained with DAPI (blue). Representative images are shown. Scale bar: 10 µm.