Table 3.

Primer sequences used for quantitative real-time PCR.

Gene	Protein	5′-3′Sequence	Reference 1	Annealing Temperature (°C)	Efficiency
β-ACTIN beta-Actin	β-Actin	CGCGAGAAGATGACCC ATTGCCAATGGTGATGAC	NM_001101	59	2.0
GAPDH glycerinaldehyd-3-phosphat-dehydrogenase	GAPDH	AGGTCGGAGTCAACGGAT TCCTGGAAGATGGTGATG	NM_002046	59	1.8
β2M beta-2- microglobulin	β2M	TGTGCTCGCGCTACTCTCTCTT CGGATGGATGAAACCCAGACA	NM_004048	59	2.0
ICAM1 Intercellular adhesion molecule 1	ICAM1	ACCATCTACAGCTTTCCGGC CAATCCCTCTCGTCCAGTCG	NM_000201.3	63	1.9
IL6 Interleukin 6	IL6	ACAGCCACTCACCTCTTCAG GTGCCTCTTTGCTGCTTTCAC	NM 000600.4	63	1.9
LMNB1 Lamin B1	Lamin B1	GCAGACTTACCATGCCAAAC TCCCTTATTTCCGCCATCTC	NM 005573.3	63	1.9
MCP1 Monocyte chemotactic Protein 1	MCP1	CTTCTGTGCCTGCTGCTCATA GGACACTTGTCGCTGGTGATT	NM 002982.3	66	2.0
p21/CDKN1A cyclin-dependent kinase inhibitor 1A	p21	GCAGACCAGCATGACAGATTTC ACCTCCGGGAGAGAGGAAAA	NM_000389.4	66	1.8
p27/CDKN1B Cyclin-dependent kinase inhibitor 1B	p27	CAGCTTGCCCGAGTTCTACT AGAAGAATCGTCGGTTGCAGG	NM_004064.4	66	2.0
p53 tumor protein p53	p53	AGATAGCGATGGTCTGGC TTGGGCAGTGCTCGCTTAGT	NM_000546.5	63	2.0

¹ Accession numbers from reference sequences taken from GenBank are shown.