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. 2020 Dec 21;12(12):3859. doi: 10.3390/cancers12123859

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RL1 cell growth effects. (a) Cell growth of glioblastoma stem cells (GSCs) measured with MTT (3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide) absorbance assay after two and four days of incubation, (b) main protein expression proteins of the mTOR pathway in cancerous and non-cancerous cells, (c) cell growth dose dependent decrease comparison measured with MTT absorbance after 4 days of incubation, (d) 4-day incubation significant difference of cell growth decrease measured with MTT absorbance of cancer GSCs compared to non-cancerous NSCs. All the plots present the mean and the standard deviation. The p-value < 0.05 was considered statistically significant in all analysis. Statistical tests performed for two variables, unpaired Student’s t-test, for more than two related variables, one-way-ANOVA. The significance of the difference between groups was described as **** p < 0.0001.

RL1 cell growth effects. (a) Cell growth of glioblastoma stem cells (GSCs) measured with MTT (3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide) absorbance assay after two and four days of incubation, (b) main protein expression proteins of the mTOR pathway in cancerous and non-cancerous cells, (c) cell growth dose dependent decrease comparison measured with MTT absorbance after 4 days of incubation, (d) 4-day incubation significant difference of cell growth decrease measured with MTT absorbance of cancer GSCs compared to non-cancerous NSCs. All the plots present the mean and the standard deviation. The p-value < 0.05 was considered statistically significant in all analysis. Statistical tests performed for two variables, unpaired Student’s t-test, for more than two related variables, one-way-ANOVA. The significance of the difference between groups was described as **** p < 0.0001.