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. 2020 Dec 21;12(12):3859. doi: 10.3390/cancers12123859

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RL1 effect on stemness and EMT protein marker expression. (a) RL1 protein expression analysis validates the inhibition of neural stem cell markers and mesenchymal transformation markers in our GSCs, (b) RL1 strongly inhibited colony formation in all GSCs in agar assays, (c) RL1 strongly inhibited migration in all cell lines except NCH644 in Boyden chamber assays. Statistical tests performed for two variables, unpaired Student’s t-test. The significance of the difference between groups was described as * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.