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. 2020 Dec 17;12(12):1454. doi: 10.3390/v12121454

Figure 2.

Figure 2

Representative images of PrPSc 2D-electrophoresis are shown. PrPSc was precipitated from brain homogenate using sarkosyl and NaPTA. Extracted proteins were separated in the first dimension on Ready-Strip IPC strips (Bio-Rad) and a pH gradient of 3–10. Following equilibration in SDS and reducing/alkylating agents, the second dimension of the gel was performed on Criterion Tris-HCL polyacrylamide precast gels (8–16%) prior to Western blotting. PrPSc was detected using the 3F4 antibody and an HRP secondary. Four strips of spots were identified at different molecular weights: 32 kDa (red), 29 kDa (green), 23k Da (blue) and 13/14 kDa (purple). (A) Non-venison exposed CJD subject; (B–D) venison exposed CJD subjects.