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. 2020 Dec 20;21(24):9726. doi: 10.3390/ijms21249726

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Representative images for the immunofluorescence staining of osteocalcin (Ocl) (a) and collagen type I (ColI) (b), expressed by BM-MSCs cultured in an osteogenic differentiation medium with or without BMP-2 and/or FGF-2 for 21 days. The Ocl and ColI were stained with FITC in green and nuclei in blue with DAPI. Immunofluorescence staining for osteocalcin (c) and collagen type I (d) was quantified using the ImageJ software. FGF-2 and BMP-2 promoted the expression of osteogenic-related proteins, which resulted in a moderately more intense fluorescence in the treated cells compared to the control cells cultured in the osteogenic differentiation medium alone.

Representative images for the immunofluorescence staining of osteocalcin (Ocl) (a) and collagen type I (ColI) (b), expressed by BM-MSCs cultured in an osteogenic differentiation medium with or without BMP-2 and/or FGF-2 for 21 days. The Ocl and ColI were stained with FITC in green and nuclei in blue with DAPI. Immunofluorescence staining for osteocalcin (c) and collagen type I (d) was quantified using the ImageJ software. FGF-2 and BMP-2 promoted the expression of osteogenic-related proteins, which resulted in a moderately more intense fluorescence in the treated cells compared to the control cells cultured in the osteogenic differentiation medium alone.