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. 2020 Dec 17;21(24):9641. doi: 10.3390/ijms21249641

Figure 1.

Figure 1

Pladienolide B (PB) inhibits SF3B1 without inducing cytotoxicity in HEK293T cells. (A) Cell viability after exposure for 16 h to PB using the Trypan Blue exclusion assay. Dig = digitonin control. (B) RT-qPCR quantitation of the RBM5 exon 5/7 alternatively spliced isoform after exposure to 5 or 100 nM PB. Fold change is shown relative to DMSO control cells. Error bars indicate SEM from at least three independent biological replicates. (C) Western blots using BAG3 or actin antibodies from cells exposed to either control or heat shock (HS) and either DMSO or 100 nM PB, as indicated.