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. 2020 Dec 17;12(12):3810. doi: 10.3390/cancers12123810

Figure 6.

Figure 6

The effect of irinotecan (IRI) and IRI with BGP-15 (IRI+BGP) treatment on skeletal muscle mitochondrial metabolic phenotyping. (A) Tibialis anterior (TA) homogenate was analyzed for citrate synthase (CS) activity as a marker of mitochondrial density. Isolated flexor digitorum brevis (FDB) fibers were utilized for mitochondrial metabolic analyses with oxidative respiration indices, i.e., (B) basal respiration, (C) adenosine triphosphate (ATP) production rate, (D) coupling efficiency, (E) spare respiratory capacity, as well as glycolytic respiration indices, i.e., (F) basal extracellular acidification rate (ECAR) and (G) ECAR metabolic potential, measured. Furthermore, Western blotting experiments were undertaken and TA homogenate was probed for (H) total poly-(ADP-ribose) polymerase-1 (PARP-1) and (I) heat shock protein-70 (HSP-70)—these data are expressed as a relative percentage of the vehicle (VEH) control group. (J) Western blot representative images are displayed alongside a Coomassie Blue representative image, which was used as the protein loading control. * = p < 0.05 compare to VEH; ^ = p < 0.05 compared to IRI; n = 4–8 for CS activity and n = 5–8 for metabolic phenotyping and Western blotting.