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Percentage (P) of HSA denaturation as a function of temperature (left). Graphical representation for calculation of the HSA denaturation temperature (right). HSA (■), HSA-Fh-NPs (▼), HSA-Co-Fh-NPs (▲), and HSA-Cu-Fh-NPs (●). HSA (2.5 µM) was dissolved in 100 mM HEPES, at pH 7.25.
