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. 2020 Dec 17;21(24):9607. doi: 10.3390/ijms21249607

Figure 3.

Figure 3

Notch signalling is needed to maintain astrocytic EAAT function. Example traces of EAAT mediated currents in response to 200 μM L-Asp in (i) DIV13 neuronal cocultured (CC) astrocyte, (ii) DIV24 CC astrocyte, (iii) DIV24 monocultured (MC) astrocyte, (iv) DIV24 CC astrocyte +DAPT from DIV0, and (v) DIV24 astrocyte +DAPT from DIV14. Cells were voltage-clamped at −80 mV and all recordings were done in the presence of 100 μM AP-5. (vi) At DIV24 there was a significantly larger EAAT mediated response in control CC astrocytes compared to MC astrocytes (p = 0.009, LME ANOVE, df = 29), as well as CC astrocytes treated with DAPT from either DIV0 or DIV14 (p = 0.028 & 0.027, for DIV0 and DIV14 respectively, LME ANOVA, df = 29). There was no difference in EAAT response in CC astrocytes treated with DAPT from DIV0 or from DIV14 after currents had been established. Recordings were taken from cells across at least three independent culture batches. * p < 0.05, ** p < 0.001.