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. 2020 Dec 19;21(24):9707. doi: 10.3390/ijms21249707

Figure 3.

Figure 3

CoA biosynthesis and protein acetylation. Mass spectrometry quantification of (A) free CoA, (B) dpCoA, (C) pantothenate, and (D) acetyl-CoA in the forebrain from Ctrl (n = 6) and Syn-Coasy (n = 6) mice (age P12–P14). * p < 0.05 (Student’s t-test). (E) Western blot analysis and (F) densitometric quantification of acetylation levels for tubulin (acTub), histone 3 (acH3), and total lysine residues (acLys) in forebrain homogenates from Ctrl (n = 8) and Syn-Coasy (n = 8) mice (age P12–P14). GAPDH was used as the loading control for acH3 and acLys, while acTub was normalized against total tubulin (Tub). No statistically significant differences were observed (one-way ANOVA). (G) Relative expression of Pank1α and β, Pank2, Pank3, Ppcs, and Ppcdc mRNAs in the Ctrl (n = 5) and Syn-Coasy (n = 5) mice forebrain. No statistically significant differences were observed (one-way ANOVA). For all charts, mean ± SD is shown.