Figure 6.

The effect of the antiasthma medication ciclesonide on CSC levels in breast cancers. (A) The mRNA levels of Sox2, Nanog, Oct4, and CD44 were assessed in ciclesonide-treated mammospheres using specific primers. β-actin was used as the loading control. * p < 0.05 vs. the control. Experiments were repeated three times. (B) Ciclesonide (10 μM) inhibited mammosphere growth. Ciclesonide-treated mammospheres were dissociated into single cells, and equal numbers of cells were cultured in 6 mm dishes. One day after plating, the cells were counted. After two and three days, the cells were counted in triplicate, and the mean value was plotted. (C) The proposed schematic suggests that ciclesonide induced GR degradation, YAP downregulation, and CSCs inhibition. GR and YAP activity contributes to CSC formation and inhibition of GR and YAP expression via siRNA suppresses CSCs formation.