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. 2020 Dec 18;21(24):9694. doi: 10.3390/ijms21249694

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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CD34+ and αSMA+ stromal cells in tumours infiltrating adipose tissue. (A–D): signet ring carcinoma with Krukenberg tumour and peritoneal dissemination. TCs/CD34+SCs are observed around neoplastic cells (A,B,D), which show PAS+ vacuoles (C) and express cytokeratin AE1/AE3 (D). (A,B): CD34 immunochemistry; haematoxylin counterstain. (C): PAS staining. (D): double immunochemistry labelling for CD34 (red) and cytokeratin AE1/AE3 (brown). (E): neoplastic glands (asterisk) of adenocarcinoma of colon infiltrating adipose tissue. αSMA cells (brown) are observed in the stroma. αSMA immunochemistry. Haematoxylin counterstain. Bar: (A–D): 20 µm; (E): 100 µm.