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Confocal microscopy images of platelets in PRP (red) incubated with coumarin-labelled PLGA-PEG NPs (green) at 2.2 mg/mL. (A) For 1 min; (B) For 5 min; (C) For 30 min. Platelets in PRP were fluorescently labelled with phalloidin-TRITC at 0.25 mg/mL and fixed using 1% formaldehyde Upper panel, 113 nm NPs; middle panel, 321 nm NPs; lower panel, 585 nm NPs. Magnification 100×, scale bar represents 5 µm.
