Skip to main content
. 2020 Dec 18;12(12):3821. doi: 10.3390/cancers12123821

Figure 3.

Figure 3

Knock-down of GRP78 blocks the entry of IGFBP-3 into the cells. IF staining of GRP78 and IGFBP-3 in cells treated with IGFBP-3, in the presence or absence of target siRNA to GRP78, or NS siRNA in (A) HS578T and (B) MDA-MB-231 cells (Original magnification 63×). (C,i) Hs578T cells treated with IGFBP-3 with and without GRP78 knocked-down were subjected to cell surface (cs) biotinylation followed by Western blot analysis. (C,ii) Quantification of Western blot results shown in (C,i). The whole blots (uncropped blots) are shown in the Supplemental Materials.