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TZM FLIM-FRET analysis in MDA-MB-231 cancer cells. (A) The representative TCSPC images of fluorescence intensity and mean lifetime map (τm) in cells treated with TZM–AF700 (A:D = 0:1) or with TZM–AF700 plus TZM–AF750 (A:D = 2:1) pseudo-color range = 300–1500 ps. Scale bar = 50 µm. (B) Comparison of fluorescent lifetime distribution in MDA-MB-231 (solid lines) and AU565 cells (dashed lines) treated with TZM–AF700 (A:D = 0:1 red), TZM–AF700 plus TZM–AF750 (A:D = 2:1 black).
