Figure 5.

Effects of ADPN on the ion currents in a single SMC in the presence of L-NNA. (Aa,Ab) Typical tracing of total outward K⁺ current recorded in a single control cell (Aa) and after ADPN addition (Ab). (Ba–Bc) Typical tracing of total outward K⁺ current recorded in a single control cell (Ba), in L-NNA (Bb), and after ADPN addition in the presence of L-NNA (Bc). (Ca,Cb) Typical tracing of total inward Ca²⁺ current recorded in a single control cell (Ca) and after ADPN addition (Cb). (Da–Dc) Typical tracing of total inward Ca²⁺ current recorded in a single control cell (Da), in L-NNA (Db), and after ADPN addition in the presence of L-NNA (Dc). All current values are normalized to cell capacitance. (E) Bar charts representing the effects of ADPN, L-NNA, and L-NNA+ADPN on the total K⁺ current outflow. (F) Bar charts representing the effects of ADPN, L-NNA, and L-NNA+ADPN on the total Ca²⁺ current inflow. Bar charts are related to the current amplitude evoked by the +50 mV step pulse, measured at the end of the stimulus for K⁺ current and at the peak amplitude for Ca²⁺ currents (Ip, peak current). Data evaluated in control condition (CTRL), 15 min after adiponectin addition to the bath solution (ADPN) and after adding adiponectin in the presence of L-NNA (L-NNA+ADPN). (E,F) One-way ANOVA with Bonferroni’s post hoc test was used for multiple comparisons. *, p < 0.05 vs. control; #, p < 0.05 vs. ADPN; §, p < 0.05 vs. L-NNA. Values are means ± SD. Current mean values are listed in Table 1.