Figure 6.

Lack of effects of ADPN on SMC electrophysiological properties and ion currents in the presence of dorsomorphin (DORS). (A) Bar charts showing the effect of ADPN and DORS+ADPN on the resting membrane potential (RMP) of the SMCs. (B) Bar charts showing the effect of ADPN and DORS+ADPN on the cell capacitance (Cm), the index of cell surface, of the SMCs. (C) Bar charts showing the effect of ADPN and DORS+ADPN on the membrane conductance (Gm), the index of membrane permeability, of the SMCs. (Da,Db) Typical tracing of total outward K⁺ current recorded in a single control cell (Da) and after ADPN addition (Db). (Ea–Ec) Typical tracing of total outward K⁺ current recorded in a single control cell (Ea), in dorsomorphin (Eb) and after ADPN addition in the presence of dorsomorphin (Ec). (Fa,Fb) Typical tracing of total inward Ca²⁺ current recorded in a single control cell (Fa) and after ADPN addition (Fb). (Ga–Gc) Typical tracing of total inward Ca²⁺ current recorded in a single control cell (Ga), in dorsomorphin (Gb) and after ADPN addition in the presence of dorsomorphin (Gc). All current values are normalized to cell capacitance. (H) Bar charts representing the effects of ADPN, DORS, and DORS+ADPN on the total K⁺ current outflow. (I) Bar charts representing the effects of ADPN, DORS, and DORS+ADPN on the total calcium current inflow. Bar charts are related to the current amplitude evoked by the +50 mV step pulse, measured at the end of the stimulus for K⁺ current and in the initial part of the stimulus for Ca²⁺ currents (Ip, peak current). Data evaluated in control condition (CTRL), 15 min after adiponectin addition to the bath solution (ADPN), and after adding adiponectin in the presence of dorsomorphin. One-way ANOVA with Bonferroni’s post hoc test was used for multiple comparisons. *, p < 0.05 vs. control; #, p < 0.05 vs. ADPN. Current mean values ±SD are listed in Table 1.