Figure 1.

Effects of chalcone derivatives and flavonoids on the inhibition of lipopolysaccharide (LPS)-primed ATP-induced pro-caspase-1 processing in differentiated THP-1 cells. THP-1 cells were seeded and differentiated by 50 nM PMA for 48 h. Then, the cells were primed with 1 μg/mL LPS for 4 h and were pre-treated with or without the indicated compound for 30 min and then treated with 5 mM ATP for another 2 h. After treatment, the concentrations of p20 caspase-1 subunit in the medium were determined. Data are expressed as mean±SEM of three to four independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001 compared with compound-free control.