Figure 5.

Effect of 11Cha1 on ATP-induced interaction between NLRP3, pro-caspase-1, and ASC. THP-1 cells were seeded and differentiated by 50 nM PMA for 48 h and then primed with 1 μg/mL LPS for 4 h. The cells were pre-treated with or without 11Cha1 (30 µM) for 30 min and then treated with 5 mM ATP for another 2 h. After treatment, immunoprecipitation and immunoblotting experiments were performed. Data are expressed as mean ± SEM of four independent determinations. One-way ANOVA by Newman Keuls post hoc test was used. The analyses of P (ANOVA) indicated P < 0.001 in all tests.