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Effect of 11Cha1 on ATP-induced pyroptosis reaction. THP-1 cells were seeded and differentiated by 50 nM PMA for 48 h and then primed with 1 μg/mL LPS for 4 h. The cells were pre-treated with or without the indicated agent for 30 min and then treated with 5 mM ATP for another 2 h. After the treatment, the LDH levels in medium was determined using LDH assay kit. Data are expressed as mean ± SEM of five independent determinations.
