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. 2020 Dec 20;12(12):3848. doi: 10.3390/cancers12123848

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) The level of DNA damage in PANC-1 and 1.2B4 cells exposed to silver nitrate (AgNO₃), metronidazole, 4-hydroxymethylpyridine, ((MTZ)₂Ag)NO₃, and ((4-OHMePy)₂Ag)NO₃ (0.0, 0.5, 0.75, 1.0, 5.0 µM) for 1 h at 37 °C. The DNA damage was determined by the alkaline comet assay. Treatment with 20 µM H₂O₂ for 10 min on ice served as a positive control. Data are expressed as mean ± SEM. * p < 0.05; ** p < 0.01, *** p < 0.001 in comparison to untreated cells (negative control). (B,C) The representative images of comets after exposure to the tested agents visualized by staining with 2 mg/mL 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) and observed under fluorescence microscope (Nikon, Tokyo, Japan) at 200× magnification.

(A) The level of DNA damage in PANC-1 and 1.2B4 cells exposed to silver nitrate (AgNO₃), metronidazole, 4-hydroxymethylpyridine, ((MTZ)₂Ag)NO₃, and ((4-OHMePy)₂Ag)NO₃ (0.0, 0.5, 0.75, 1.0, 5.0 µM) for 1 h at 37 °C. The DNA damage was determined by the alkaline comet assay. Treatment with 20 µM H₂O₂ for 10 min on ice served as a positive control. Data are expressed as mean ± SEM. * p < 0.05; ** p < 0.01, *** p < 0.001 in comparison to untreated cells (negative control). (B,C) The representative images of comets after exposure to the tested agents visualized by staining with 2 mg/mL 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) and observed under fluorescence microscope (Nikon, Tokyo, Japan) at 200× magnification.