Figure 3.

Sensitivity of TaqMan capB and lef duplex in LightCycler 2.0. Ten-fold serial dilutions of an Ames strain carrying pXO1 and pXO2 plasmids were tested in triplicate to establish the limit of detection of capB and lef markers. capB and lef were consistently detected with ~2 × 10¹ and ~9 × 10¹ GE of pXO2 (Panel (A)) and pXO1 (Panel (B)), respectively. The standard curve plots displayed R² and slopes values as follows: capB, R² = 0.976, slope = −3.4404; lef, R² = 0.9594, slope = −3.486.