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. 2020 Dec 21;9(12):1074. doi: 10.3390/pathogens9121074

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Evaluation of 4-color qPCR assay in QuantStudio. A collection of B. anthracis strains previously known to carry pXO1 and pXO2 produced consistent amplification of Ba-1 (red), capB (black) and lef (blue) markers (Panel (A)). The GI4 marker (grey) was specifically amplified in Bcbva backgrounds (Panel (B)) that were also strongly positive for capB (black) and lef (blue). Panel (C) illustrates amplification of Ba-1 (red) and lef (blue) markers in capB-negative B. anthracis isolates. Conversely, lef-negative B. anthracis only amplified Ba-1 (red) and capB (black) markers (Panel (D)).