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. 2020 Dec 21;9(12):1074. doi: 10.3390/pathogens9121074

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Evaluation of Ba-1-GI4 duplex PCR in LightCycler 2.0. A collection of B. anthracis and Bcbva strains were evaluated with the Ba-1 and GI4 TaqMan duplex PCR using 1 ng of genomic DNA. All B. anthracis strains tested (blue, panel (A)) showed clear amplification with the Ba-1 marker in the 560 channel, which detects signal from the VIC-labeled Ba-1 probe. Conversely, signal from GI4 was only observed for Bcbva (red, panel (B)) in the 530 channel, which detects signal from the FAM-labeled GI4 probe.