Table 4.
Evaluation of B. cereus sensu lato panel with LC2 and QuantStudio 7 TaqMan assays. Average cycle thresholds are shown.
| LightCycler 2.0 | QuantStudio 7 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Species | ID | Ba-1 | capB | lef | GI4 | Ba-1 | capB | lef | GI4 |
| Ba a Ames | UF00738 | 25.14 | 20.37 | 20.95 | Und. | 24.35 | 23.67 | 21.90 | Und. |
| Ba Vollum 1 | UF00980 | 24.69 | 32.12 | 32.45 | Und. | 25.85 | 37.52 | 36.08 | Und. |
| Ba Ames | UF01105 | 25.49 | 20.06 | 20.49 | Und. | 23.09 | 22.25 | 20.60 | Und. |
| Ba Ames | UF01106 | 26.17 | 20.46 | 20.89 | Und. | 24.69 | 23.73 | 21.92 | Und. |
| Ba Vollum (A4) | UF01096 | 25.36 | 20.68 | 20.73 | Und. | 24.55 | 23.84 | 21.83 | Und. |
| Ba WNA (A1.a) | UF01137 | 25.02 | 19.95 | 20.40 | Und. | 23.83 | 23.06 | 21.26 | Und. |
| Ba | UF00175 | 25.31 | 20.84 | 20.77 | Und. | 24.74 | 24.13 | 21.91 | Und. |
| Ba | UF01063 | 23.85 | 18.90 | 19.31 | Und. | 23.08 | 21.98 | 20.17 | Und. |
| Ba | UF01075 | 24.36 | 20.06 | 20.33 | Und. | 24.73 | 23.73 | 21.89 | Und. |
| Ba | UF01052 | 25.53 | 20.12 | 19.99 | Und. | 24.23 | 23.25 | 21.15 | Und. |
| Ba | UF00049 | 25.93 | 20.11 | 19.80 | Und. | 24.40 | 23.31 | 21.02 | Und. |
| Ba | UF01135 | 23.69 | 18.50 | 18.79 | Und. | 22.35 | 21.28 | 19.65 | Und. |
| Ba | UF00438 | 23.82 | 17.15 | 17.84 | Und. | 21.75 | 20.31 | 18.89 | Und. |
| Ba | UF00603 | 23.73 | 17.50 | 18.24 | Und. | 21.86 | 20.97 | 19.48 | Und. |
| Ba | UF00727 | 23.83 | 18.26 | 17.94 | Und. | 22.40 | 22.17 | 19.86 | Und. |
| Ba | UF00910 | 23.99 | 18.62 | 18.94 | Und. | 22.51 | 21.46 | 19.77 | Und. |
| Ba | UF00926 | 23.39 | 18.01 | 18.34 | Und. | 21.98 | 21.25 | 19.42 | Und. |
| Ba | UF00930 | 23.08 | 18.78 | 18.64 | Und. | 21.45 | 21.28 | 19.22 | Und. |
| Ba | UF00965 | 23.69 | 17.97 | 18.48 | Und. | 22.09 | 20.99 | 19.49 | Und. |
| Ba Sterne | UF01114 | 26.96 | Und. | 22.69 | Und. | 26.21 | Und. | 23.58 | Und. |
| Ba | UF00839 | 23.79 | Und. | 18.93 | Und. | 23.39 | Und. | 20.47 | Und. |
| Ba | UF00878 | 24.39 | Und. | 19.38 | Und. | 23.00 | Und. | 20.05 | Und. |
| Ba | UF00232 | 25.35 | Und. | 20.35 | Und. | 24.59 | Und. | 21.26 | Und. |
| Ba | UF00242 | 23.95 | 18.70 | Und. | Und. | 22.95 | 21.97 | Und. | Und. |
| Ba | UF00502 | 24.59 | 19.71 | Und. | Und. | 23.65 | 22.89 | Und. | Und. |
| Ba | UF00621 | 22.90 | 17.35 | Und. | Und. | 21.35 | 20.64 | Und. | Und. |
| Bcbva | Bc0001 | Und. | 19.33 | 20.01 | 21.22 | Und. | 20.75 | 19.50 | 20.16 |
| Bcbva | Bc0002 | Und. | 17.81 | 18.54 | 18.53 | Und. | 20.47 | 19.27 | 19.83 |
| Bcbva | Bc0007 | Und. | 17.56 | 18.39 | 17.60 | Und. | 20.36 | 19.12 | 19.56 |
| Bcbva | Bc0009 | Und. | 20.59 | 21.31 | 20.97 | Und. | 23.07 | 21.92 | 22.46 |
| Bcbva | Bc0011 | Und. | 17.91 | 18.74 | 18.90 | Und. | 20.92 | 19.77 | 20.14 |
| Bt b 97-27 | Und. | Und. | Und. | Und. | Und. | Und. | Und. | Und. | |
| Bt NRS 996 | Und. | Und. | Und. | Und. | Und. | Und. | Und. | Und. | |
| Bt AD-1 | Und. | Und. | Und. | Und. | Und. | Und. | Und. | Und. | |
| Bt HD-1 | Und. | Und. | 38.30 | Und. | Und. | Und. | 37.36 | Und. | |
| Bt HD522 | Und. | Und. | Und. | Und. | Und. | Und. | Und. | Und. | |
| Bc c FDA 5 | Und. | Und. | Und. | Und. | Und. | Und. | Und. | Und. | |
| Bc Gibson 971 | Und. | Und. | Und. | Und. | Und. | Und. | Und. | Und. | |
| Bc NRS 201 | Und. | Und. | Und. | Und. | Und. | Und. | Und. | Und. | |
| Bc G9241 | Und. | Und. | 20.12 | Und. | Und. | Und. | 16.22 | Und. | |
a Ba = Bacillus anthracis, b Bt = Bacillus thuringiensis, c Bc = Bacillus cereus. Und. = Undetermined, no amplification was detected.