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. 2020 Dec 21;12(12):3867. doi: 10.3390/cancers12123867

Figure 2.

Figure 2

Functional assays aiming to evaluate LINC00518 expression induced by cell cycle in 92.1 cells. (A) Expression of LINC00518 during early response to serum-induced cell cycle activation. (B) Expression of LINC00518 at 24 h after serum supplement (cell cycle activation) and serum deprivation (cell cycle arrest). (C) Evaluation of cell viability after U0126 treatment of 92.1 cells through MTT assay; the effects of two concentrations were tested, showing that 25 µM induced the most significant decrease in cell viability in U0126-treated cells compared to control cells (DMSO). (D) Expression of LINC00518 in U0126-treated cells compared to control cells (DMSO). Each experiment was performed in three in vitro replicates. *: p-value < 0.05; **: p-value < 0.005; ***: p-value < 0.0005.