Figure 3.
ASFV-specific IgG (A) and IgA (B) titers found in the blood of pigs at the time of Ken06.Bus challenge, and ASFV-specific IgG1/IgG2 ratios (C) and IFNγ-secreting specific T cells found in peripheral blood mononuclear cells (PBMCs) in vitro stimulated with 106 HAU/mL of BA71, Ken06.Bus, or Georgia2007/1. Additionally, PBMCs were stimulated with 105 HAU/mL of Georgia2007/1 (D) at this same time point; any sample scoring ≥ 300 spots/106 PBMCs received a score of 300, which was considered the limit of our assay resolution. ASFV-specific antibodies and T-cells were measured by ELISA and ELISPOT (IFN-ϒ secreting cells), respectively.