Table 3.
List of conserved ASFV B-cell epitopes predicted with a structure-based approach.
| Epitope | Protein (ORF) |
Protein ID 1 | Mean Flex. 2 | Mean RSA 3 |
Sus scrofa Hit (id %) 4 |
Microbiota Hit (id %) 4 |
|---|---|---|---|---|---|---|
| KPHQSKPILTDENDTQRT | p72 (B646L) | E0WMM0 | 1.7 | 50.1 | XP_020932317.1 (38.9%) | WP_169883328.1 (50.0%) |
| NIQTAGKQDITPITD | p72 (B646L) | E0WMM0 | 1.8 | 56.8 | XP_020933726.1 (66.7%) | WP_001886209.1 (53.3%) |
1 PDB entry 6L2T did not have a reference sequence tied to UniProt. However, both epitopes are found in Georgia 2007/1 p72 protein and UniProt ID for this entry is provided. 2 Values of flexibility (arbitrary units) and 3 RSA (%) calculated as explained in Materials and Methods. 4 Hit reference provided as NCBI accession number; its percentage of identity to the predicted epitope sequence is provided in parenthesis. Underlined sequences indicate shared residues with conserved and IEDB-validated epitopes from Table 1.