Fig. 4.

UVB pre-treatment effects the location of keratinocytes within the skin equivalent model.

Skin equivalent models were generated with 20% labelled or UVB pre-treated labelled cells and their location within the different strata of the fully differentiated skin equivalent model as well as their G6PD activity were assessed.

A) IF image of cell tracker (white) labelled keratinocytes within the skin equivalent model, basal, low & high suprabasal strata indicated by pink, orange and green contours respectively. Labelled basal cells are indicated by arrow heads. B) Pie chart depicting the distribution of all cells to the epidermal strata and the distribution of UVB pre-treated, labelled cells to the strata (red contour pie chart). C) Violin plots of G6PD activity staining. Left: activity plot of non-labelled cells (blue) in comparison to labelled untreated cells (red) within the same skin equivalent. Right: activity plot of non-labelled cells (blue) and labelled UVB pre-treated cells within the same skin equivalent. D) Violin plots of G6PD activity staining of UVB pre-treated labelled cells (red) and their non-irradiated neighboring cells (blue) within the different strata of the skin equivalent model. For C and D the mean values are indicated by black bars with subscripted numeric values. Asterisks indicate statistical differences (****p < 0.0001; ns-no significant difference; Student's t-test calculated with R).