Fig. 3.

Bone marrow TTP deficiency increased macrophage response to LPS stimulation, and resulted in multi-organ damage and systemic inflammation. (A–B) Residential peritoneal macrophages were obtained from mice at the experimental endpoint. The cells were treated with LPS (50 ng/ml) for 6 h. Total mRNA was extracted for qRT-PCR to measure the expression of inflammatory cytokine TNFalpha (A) and IL6 (B). Data were expressed as mean ± SEM, (N = 3 mice for each group). **p < 0.01. Representative photomicrographs showing H&E staining in the (C) liver (20x), (D) lung (20x), and (E) spleen (10x) in wild-type recipient mice (right panels) and TTP^−/− recipient mice (left panels). Scale bar 100 μm. (F) Serum TNFalpha concentrations were determined by ELISA. Data were analyzed by Student's t-test. Data were presented as the mean ± SEM (N = 6 mice each group). ***p < 0.001.