Fig. 4.

Microarray analysis revealed that bone marrow TTP deficiency in LDLR^−/−mice modulated the expression of inflammatory genes, oxidative stress regulators, and lipid metabolism related genes. Mouse liver RNAs were isolated from LDLR^−/− mice transplanted with WT or TTP^−/− bone marrow cells and fed Western diet for 12 weeks and subjected to Affymetrix microarray analysis. N = 4/group. (A) Heat maps of 236 genes that are significantly changed by more than 2 folds and corrected P-value cut-off:0.05. See Supplementary Fig. S6 for detailed gene list. (B) Heat maps of 27 hepatic genes involved in inflammation and lipid metabolism. Color-coding indicates increased gene expressions in red and decreased gene expressions in green. The columns and rows in the heat maps represent samples and genes, respectively. (C) The network in the ingenuity pathway analysis (IPA) from top regulated pathway “inflammatory response” were shown. Genes that are significantly upregulated by bone marrow TTP deficiency are boxed. The color intensity indicates the fold change expression of genes (red representing upregulation and green representing downregulation). (D) The network in the IPA from top regulated pathway “Fatty acid metabolism” were shown. Gene that is significantly downregulated by bone marrow TTP deficiency is boxed. The color intensity indicates the fold change expression of genes (red representing upregulation and green representing downregulation). (E) qPCR analysis of liver mRNA was used to verify the results of selected genes from microarray data. Data were presented as the mean ± SEM. *p < 0.05. Microarray data was deposited at NIH-GEO dataset “GEO accession GSE126481”. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)