Fig. 5.

Bone marrow TTP deficiency increased mitochondrial reactive oxygen species (mtROS) generation. (A) Venn diagram showed some of the mitochondrial biology related genes from mouse mitocarta gene list which is significantly changed in BM TTP^−/− mice based on the microarray data. The mitocarta gene list was collected from the MIT Broad Institute website which includes all the nucleus and DNA encoded mitochondrial genes. (B) Mitochondrial ROS production. BMDMs were generated from bone marrow cells isolated from femurs and tibias of TTP^−/− mice and WT littermates. Then, cells were treated with H₂O₂ (250 μM) for 4 h, then loaded with 5 μM of MitoSOX Red Mitochondrial Superoxide indicator (Life technologies) for 20 min and flow cytometry analysis was performed to quantify mtROS (n = 3). **P < 0.01; ***P < 0.001, vs. control. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)