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. 2020 Jul 24;37:101653. doi: 10.1016/j.redox.2020.101653

Fig. 2.

Fig. 2

OGG1 affects the level of H4R3me2s modification. (A) OGG1 and the H4R3me2s′ occupancy of 8-oxoG-containing DNA in NEs. NEs (50 μg per sample) from Hela cells were incubated with G (left lane) or 8-oxoG oligonucleotides (right lane) for 5 min, and protein DNA complexes were pulled down using magnetic streptavidin beads. The washed pellets were subjected to SDS-PAGE and immunoblotting using antibodies against OGG1 or H4R3me2s. (B) The levels of H4R3me2s modification in WT and OGG1−/− MEFs were determined via Western blot analysis. (C) Whole-cell lysates of HEK293T cells transfected with OGG1 siRNA or scrambled siRNA (Scr) were immunoblotted for H4R3me2s and H4. Tubulin was used as a loading control. (D) Whole-cell lysates of HEK293T cells transfected with pcDNA3.1-OGG1 or the control vector were immunoblotted for H4R3me2s and H4. Tubulin was used as a loading control. (E) The level of H4R3me2s modification in WT and OGG1−/− MEFs treated with or without 1 mM H2O2 was detected by Western blotting. Tubulin was used as a loading control.