Table 1.

Nanoplastics induced Key Events (KE) and Adverse Outcomes (AO) summary and their relative distribution in published literature. *Sum of percentages is over 100% because multiple publications reported multiple KEs and AOs.

Title	Category	Biological level	AOP wiki ID	NPs type	Organism	Reference	Percentage in the literature (%)*
Increase, ROS	Key event	Molecular	KE 1364	PS	D. magna	[55]	19.4%
					Danio rerio	[48]
					Monogonont Rotifer (Brachionus koreanus)	[26]
					Oyster gametes (Crassostrea gigas)	[75]
					Human	[71,104]
					Caenorhabditis elegans	[95]
Oxidative stress	Key event	Molecular	KE 1392, KE 1088	PS, PC	D. magna	[55]	45.2%
					Fathead minnow (Pimephales promelas)	[39]
					Danio rerio	[48]
						[101]
						[15]
					Daphnia pulex	[93]
					Caenorhabditis elegans	[13]
					Oyster gametes (Crassostrea gigas)	[75]
					Monogonont Rotifer (Brachionus koreanus)	[26]
					Mussel (Mytilus galloprovincialis)	[64]
						[54]
					Human	[85]
						[14]
						[60]
Activation of oxidative stress pathway	Key event	Molecular	KE 1238, KE 1279	PS	D. magna	[55]	9.7%
					Mussel (Mytilus galloprovincialis)	[54]
					Monogonont Rotifer (Brachionus koreanus)	[26]
Lipid peroxidation	Key event	Molecular	KE: 1151	PS	D. magna	[55]	9.7%
					Mussel (Mytilus galloprovincialis)	[64]
					Danio rerio	[15]
DNA damage	Key event	Cellular	KE 1669	PS	Mussel (Mytilus galloprovincialis)	[64]	6.5%
					Human	[60]
Lysosome disruption	Key event	Cellular	KE 898	PS	Mussel (Mytilus galloprovincialis)	[54]	9.7%
					Mouse	[70]
					Human	[74]
Mitochondrial dysfunction	Key event	Cellular	KE 1483	PS	D. magna	[55]	13.0%
					Mussel (Mytilus galloprovincialis)	[54]
					Mouse	[70]
					Human
					Human	[71,104]
Acetylcholinesterase (AchE) inhibition	Key event	Cellular	KE 12	PS	Danio rerio	[48]	9.7%
					Caenorhabditis elegans	[13]
					Mussel (Mytilus galloprovincialis)	[64]
Phase I metabolism enzymes activation	Key event	Molecular	KE 1386, KE 850	PS	Daphnia pulex	[86]	6.5%
					Mussel (Mytilus galloprovincialis)	[64]
Pro-inflammatory cytokines activation	Key event	Cellular	KE 87, KE 1493	PS	Danio rerio	[10]	19.4%
					Mussel (Mytilus galloprovincialis)	[64]
					Human	[14]
						[83]
						[88]
						[90]
Apoptosis	Key event	Cellular	KE 1262	PS	Mussel (Mytilus galloprovincialis)	[54]	16.1%
					Mouse	[70]
					Human
					Human	[85]
						[71,104]
						[89]
Decrease of neuronal network function	Key event	Organ	KE 386	PS	Danio rerio	(Brun, van Hage et al., 2019)	6.5%
					Caenorhabditis elegans	[13]
Inflammation; Infiltration, Inflammatory cells	Key event	Tissue/Cellular	KE 1633, KE 901	PS, PC	Danio rerio	[10]	13.0%
						[15]
					Rat	[90]
					Fathead minnow (Pimephales promelas)	[39]
Bradycardia	Key event	Organ	KE 444	PS	Danio rerio	[101]	6.5%
						[100]
Accumulation, Liver lipid	Key event	Organ	KE 455	PS	Danio rerio	[15]	3.2%
Growth inhibition	Adverse outcome	Individual	KE 1521 KE 1467	PS	algae (S. obliquus)	[9]	9.7%
					Monogonont Rotifer (Brachionus koreanus)	[26]
					Caenorhabditis elegans	[13]
Decreased body size/length	Adverse outcome	Individual	KE 315, KE 864	PS	D. magna	[9]	13.0%
					Danio rerio	[48]
					Caenorhabditis elegans	[13]
						[95]
Increase, Mortality	Adverse outcome	Individual, Population	KE 350, KE 351, KE 342	PS	D. magna	[55]	3.2%
Locomotor activity, decreased	Adverse outcome	Individual	KE 1389	PS	D. magna	[55]	22.6%
					Danio rerio	[48]
						(Brun, van Hage et al., 2019)
						[100]
					Caenorhabditis elegans	[13]
						[95]
					Rat	[82]
Impaired, Fertility	Adverse outcome	Individual	KE 406, KE 78	PS	Monogonont Rotifer (Brachionus koreanus)	[26]	3.2%
Impaired, Development	Adverse outcome	Individual	KE 577, KE 339	PS	Danio rerio	[101]	13.0%
						[48]
					Caenorhabditis elegans	[13]
					D. magna	[9]