Figure 2.

Effect of sera from SCD on EB integrity. Confluent HUVEC monolayers were incubated with sera (20% v/v) from SCD patients (n=20) or healthy volunteers (n=10) and normalized resistance was recorded at 4,000 Hz. Each line represents the mean ± SEM in the specified time points from experiments comparing (A) patients and healthy volunteers; or (B) patients subgrouped by total levels of heme in serum. Differences were compared using the Mann-Whitney test. Statistical significant differences are indicated by colored asterisks on each timepoint. *P= 0.01 to 0.05; **P<0.01.