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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Curr Opin Chem Biol. 2020 Jun 28;60:1–9. doi: 10.1016/j.cbpa.2020.04.018

Figure 1|. Major challenges to single-cell protein analysis by mass-spectrometry and their solutions.

Figure 1|

Single-cell proteomics by mass-spectrometry faces three main challenges: (i) Delivering enough copy number of ions to MS detectors to afford accurate quantification; (ii) Reliable amino acid sequence determination of quantified ions; (iii) Scaling the analysis to many thousands of single cells at affordable cost. Each of these challenges is addressed by one or more solutions. These solutions are highly synergistic and thus depicted as interlocking puzzle pieces with a few prominent examples from each category.