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. 2020 Dec 14;11:583661. doi: 10.3389/fmicb.2020.583661

FIGURE 2.

FIGURE 2

Phage cocktails show synergy compared to individual phage and their efficacy is MOI dependent. Growth kinetics of M. smegmatis cultures treated with (A) 3-phage cocktail (D29, Che7, and TM4) and (B) 5-phage cocktail (D29, TM4, Che7, PDRPv, and PDRPxv) at MOI of 10 for each phage against a non-treated control. Growth kinetics of M. smegmatis cultures treated with a 5-phage cocktail at the MOI of (C) 1, (D) 0.1, and (E) 0.01 for each phage in the phage cocktail. The experiments with MOI of 10 with 3- and 5-phage cocktails were repeated twice and the representative graphs are shown here. Red arrow represents the time point phage solution was added to the cultures. Multiple t-test with Holm-Sidak method was used to estimate the statistical significance (p < 0.05). α represents a significant difference (p < 0.05) between control and three-phage cocktail (D29, TM4, and Che7) treated (log phase) for all time points between 21 and 105 h. β represents a significant difference (p < 0.05) between control and five-phage cocktail (D29, TM4, Che7, PDRPv, and PDRPxv) treated (log phase) for all time points between 18 and 346 h. χ represents a significant difference (p < 0.05) between control and five phage cocktail treated (MOI 1, log phase) for all time points between 18 and 270 h. δ represents a significant difference (p < 0.05) between control and five phage cocktail treated (MOI 0.1, log phase) for all time points between 18 and 174 h.