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. 2020 Dec 14;11:610963. doi: 10.3389/fimmu.2020.610963

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Copyright © 2020 Xiao, Liu and Guo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The distribution of T cells, GARP staining, and Masson trichrome staining in endometriotic lesions of the two groups of mice in Experiment 1. (A) Representative immunohistochemical staining of FOXP3, T-bet, GATA-3, RORγT, GARP, along with Masson trichrome staining in the two groups. All magnifications were ×400. Scale bar = 50 µm. (B) Kinetic changes in the density of FOXP3+ (Treg), T-bet+ (Th1), GATA-3+ (Th2), and RORγT+ (Th17) cells, the staining level of GARP, and the percentage of fibrotic content in the two groups. The values show the means ± SDs. *p < 0.05; **p < 0.01; ***p < 0.001; NS, not statistically significant (p > 0.05) for the difference between the testing group and the Control group (by Wilcoxon’s test). n = 7 for each group in 1 and 2 weeks; n = 10 for each group in 5 weeks. One mouse in the PD group died of internal bleeding at 1 week after induction. IC, isotype control; PD, platelet depletion; GARP, glycoprotein A repetitions predominant.