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. 2020 Aug 11;15(1):142–153. doi: 10.1111/irv.12784

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© 2020 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Histopathological lesions and immunohistochemical detection of influenza virus nucleoprotein in nasal turbinates and trachea at 5 dpi. Haematoxylin and eosin (HE) staining of tissues and immunohistochemical (IHC) detection of influenza virus nucleoprotein (NP) showed a moderate fibrinosuppurative rhinitis and lymphoplasmacytic tracheitis observed in group V2, characterised by increased number of inflammatory infiltrates and epithelial damage than the V1 (vaccine 1) group and the non‐vaccinated control (NVC) group. Viral NP immunolabelling observed in respiratory and olfactory epithelium (arrowhead) and glandular epithelium (arrow) of the trachea and nasal turbinates in all groups. Magnification 10x