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. 2020 Dec 14;11:601907. doi: 10.3389/fmicb.2020.601907

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Copyright © 2020 Napieralski and Roden.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Conceptual cartoon of the generation of organotrophic incubations. The solids were isolated from the initial lithotrophic incubations for both live inoculated (DIO-Ox) and uninoculated control experiments (DIO-Cont) and resuspended in fresh Luquillo artificial river water (L-AGW). The resultant slurries were divided into duplicate reactors, and either reinoculated (Inoc) or left as a sterile control and stoppered. 1.0 mM filter sterilized glucose (Gluc) was added to each reactor via needle and syringe.