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. 2020 Dec 14;11:603144. doi: 10.3389/fgene.2020.603144

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Copyright © 2020 Lin, Tang, Zhao, Zhao, Zhang, Wei and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FoxO1 suppresses pre-adipocyte differentiation. (A) Overexpressed FoxO1 in pre-adipocytes. (B) Detected adipogenic label genes in pre-adipocytes after FoxO1 overexpression. (C,D) After 8-day differentiation inducer medium (DIM) stimulation, oil red O staining (C), and triglyceride assay (D). (E) Specific interference of FoxO1 by siRNA in pre-adipocytes. (F) After interference of FoxO1, the expression level of adipogenesis label genes in pre-adipocytes was detected. (G,H) After 8-day differentiation inducer medium (DIM) stimulation, oil red O staining (G), and triglyceride assay (H). *p < 0.05, **p < 0.01, and ***p < 0.001.