FIGURE 3.

FIS regulated the activity of AMPK and NF-κB and inflammatory responses in BLM-induced mice. One week after 2.5 mg/kg of BLM treatment, mice were orally administered with FIS (100 mg/kg) once every other day for 3 weeks. Protein expressions of TGF-β, p-Smad3/Smad3, p-AMPK/AMPK and p-p65/p65 in lung tissue were detected by Western blotting analysis (A, B). The levels of IL-1β, IL-6, TGF-β, MMP-9 and TNF-α in BALF were detected by ELISA assay. Data were expressed as mean ± SD (n ≥ 3). ^* p < 0.05, ^** p < 0.01. NS, non-significant.