FIGURE 5.

FIS relieved BLM-mediated senescence in A549 cells via regulating AMPK/NF-κB signaling pathway. A549 cells were pretreated with 10 μM Compound C (CC) and subsequently incubated with/without BLM (5 μg/ml), FIS (10 μM) for 3 days. SA‐β‐Gal staining was shown under ×100 magnification (A, B). Protein expressions of p16, p21, p-AMPK/AMPK and p-p65/P65 were detected by Western blotting analysis (C, D). Data were shown as mean ± SD (n ≥ 3). ^* p < 0.05, ^** p < 0.01.