Table 1.
List of primers used to amplify c3 and 18s ribosomal RNA genes in gilthead sea bream.
| Name | Sequence (5’-3’) | Temp (°C) | Efficiency (%) | R2 |
|---|---|---|---|---|
| Complement gene | ||||
| c3.1.1Fwd | GATCAGGTTGGAGAACCCAG | 60 | 101.4 | 0.99 |
| c3.1.1Rev | GACCCTGTCTCCTTCAGAAC | |||
| c3.1.2Fwd | GTTAAGGTCACTGAGTGATGC | 60 | 92.7 | 0.99 |
| c3.1.2Rev | GCTCACTAAAGTGCCTTTACTC | |||
| c3.1.3Fwd | GCTTTGAAACATAAGAACTGCACA | 60 | 91.6 | 0.99 |
| c3.1.3Rev | TCCATTTGCCAGAGGTTAATTTGT | |||
| c3.1.4Fwd | GATGAACAGAGTCAGGCGTAC | 62 | 94.3 | 1 |
| c3.1.4Rev | CATCCGTGTTGCGTGTACTTC | |||
| c3.2Fwd | GACCTGAGGGACACAGTCAG | 60 | 97.6 | 0.99 |
| c3.2Rev | CACGGTGGACTTGCTGAAGT | |||
| Reference gene | ||||
| 18s Fwd | TGACGGAAGGGCACCACCAG | 60 | 90.3 | 1 |
| 18s Rev | AATCGCTCCACCAACTAAGAACGG | |||
The annealing temperature, primer pairs efficiency (%) and the linearity R2 of the standard curve are indicated.