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Dilution ratio and the concentration (copies/ml) of SARS-CoV-2 pseudovirus RNA standards used in each assay
| qRT-PCR and OSN-qRT-PCR | ddPCR | |||
|---|---|---|---|---|
| Dilution ratio | Concentration (copies/ml) | Dilution ratio | Concentration (copies/ml) | |
| S0 | 0 | 20,000 | 0 | 150,000 |
| S1 | 5 | 4000 | 5 | 30,000 |
| S2 | 10 | 2000 | 20 | 7500 |
| S3 | 20 | 1000 | 100 | 1500 |
| S4 | 40 | 500 | 300 | 500 |
| S5 | 100 | 200 | 600 | 250 |
| S6 | 200 | 100 | 1000 | 150 |
| S7 | 400 | 50 | – | – |
| S8 | 1000 | 20 | – | – |
