. 2020 Dec 28;17:197. doi: 10.1186/s12985-020-01467-y

Table 5.

The results of qRT-PCR, OSN-qRT-PCR and ddPCR and further clinical information of 34 clinical samples from 24 acute phase COVID-19 patients

Sample ID	Results of qRT-PCR (Ct Value)		Results of OSN-qRT-PCR(Ct Value)		Results of ddPCR (copies/ml)		Specimen type	Initial official reports by OSN-qRT-PCR	Judgment by N-PCR	Judgment by ddPCR
Sample ID	ORF1ab	N	ORF1ab	N	ORF1ab	N	Specimen type	Initial official reports by OSN-qRT-PCR	Judgment by N-PCR	Judgment by ddPCR
1	27.20	26.94	15.00	16.68	129,415	1287.00	Throat swab	P	P	P
2	35.04	35.35	22.60	22.80	428.90	572.00	Throat swab	P	P	P
3	–	–	37.11	37.22	–	–	Throat swab	N	N	N
4	34.46	34.95	21.74	22.76	500.50	715.00	Throat swab	P	P	P
5	31.31	30.66	17.39	19.21	5148.00	10,224.50	Throat swab	P	P	P
6	28.02	27.38	14.65	15.25	74,360.0	364.45	Throat swab	P	P	P
7	–	–	38.21	–	–	–	Throat swab	N	N	N
8	32.61	32.70	18.17	19.96	2860.00	3503.50	Throat swab	P	P	P
9^a	40.32	–	23.50	24.64	228.80	429.00	Throat swab	N	P	S
10	35.50	35.10	23.40	23.96	715.00	715.00	Throat swab	P	P	P
11	35.08	33.09	23.60	22.14	715.00	2145.00	Throat swab	P	P	P
12	26.58	26.76	12.52	13.95	215,930	4336.0	Throat swab	P	P	P
13^a	40.83	–	28.75	26.19	157.30	228.80	Throat swab	N	P	N
14	34.00	33.96	20.84	23.19	1930.50	1716.00	Throat swab	P	P	P
15	34.37	34.38	21.70	23.10	2216.50	1144.00	Throat swab	P	P	P
16	–	–	–	38.56	–	–	Throat swab	N	N	N
17	34.33	34.27	22.08	20.28	1215.50	2502.50	Throat swab	P	P	P
18^a	–	40.21	28.67	26.88	300.30	786.50	Throat swab	N	P	S
19	29.29	29.42	18.21	18.77	31,460	42,900	Sputum	P	P	P
20	–	–	36.11	–	572.00	1787.50	Sputum	N	N	P
21	29.89	29.25	18.30	19.36	13,799	30,530	Sputum	P	P	P
22	30.45	29.82	21.82	23.99	–	–	Sputum	P	P	N
23	26.79	26.94	15.78	17.50	23,595.0	14,657.50	Sputum	P	P	P
24^a	40.81	–	35.98	36.21	–	1430.0	sputum	N	N	N
25^a	40.21	–	27.88	25.63	643.50	1215.50	Sputum	N	P	P
26	31.10	30.32	22.56	21.94	9509.50	3789.50	Sputum	P	P	P
27	27.85	27.45	20.67	20.34	84,370.0	170,170.0	Sputum	P	P	P
28	29.89	28.22	22.10	21.31	643.50	–	Sputum	P	P	N
29^a	–	40.22	16.62	17.08	715.0	715.0	Blood	N	P	P
30	–	–	–	37.90	–	–	Blood	N	N	N
31	35.08	34.73	21.57	22.83	500.50	715.00	Anal swab	P	P	P
32^a	–	40.55	25.20	24.68	214.50	–	Anal swab	N	P	N
33^a	–	40.06	24.00	25.68	–	250.25	Anal swab	N	P	N
34	–	–	26.66	27.22	307.45	–	Anal swab	N	P	N

^aOne or two of the target genes had a Ct value happen to fall in the gray zone (Ct value: 37–40), and samples were retested by qRT-PCR. At last, these samples were considered as negative with Ct value > 40 (both targets genes) by qRT-PCR

P, positive; N, Negative; S, suspect; GGO, ground glass opacities image