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. 2020 Sep 13;66(6):547–554. doi: 10.1262/jrd.2020-071

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©2020 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 2. — Mitochondrial DNA copy number (Mt-number) per granulosa cell (GC) of young (solid line) and aged (dotted line) cows. GCs were collected from the antral follicles (day 0), and the surviving GCs were selected at day 1 and cultured under high oxygen tension for 2 days. GCs immediately after collection (day 0), surviving GCs separated on day 1, and GCs cultured for 2 days after re-seeding (days 3 and 4) were subjected to mitochondrial DNA copy measurement. * indicates significant (P < 0.05) differences between young and aged cows.