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. 2020 Dec 23;4(2):e202000804. doi: 10.26508/lsa.202000804

Figure S4. Sequencing confirmation of CRISPR/Cas9 generated FAM83F cell lines.

Figure S4.

(A) Reference DNA sequence of the CRISPR/Cas9 strategy to knock-in a GFP sequence into the N-terminus of the FAM83F locus and confirmation sequencing of the transition zone between GFP and FAM83F exon 1 in the HCT116 GFP/GFPFAM83F cell lines. (B) Reference DNA sequence of FAM83F exon 2 and sequencing confirming alterations in the HCT116 FAM83F−/− and DLD-1 FAM83F−/− cell lines. These nucleotide changes predict a large deletion in the FAM83F protein sequence in HCT116 FAM83F−/− cells, and the presence of premature stop codons in both detected FAM83F alleles for DLD-1 FAM83F−/− cells.